香橼、佛手中黄酮类成分含量测定及HPLC特征图谱研究

姜艳艳; 张乐; 刘娟; 孟祥娟; 周海燕; 刘斌; 李军祥

doi:10.11653/syfj2013200103

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香橼、佛手中黄酮类成分含量测定及HPLC特征图谱研究

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- 香橼、佛手中黄酮类成分含量测定及HPLC特征图谱研究
- Determinnaiton of Flavonoids and Study on HPLC Characteristic Spectrum of Citrus Fructus and Citrus Sarcodactylis Fructus
- 中国实验方剂学杂志 2013年19卷第20期页码：103-107
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重大新药创制项目(2010ZX09102-211)
- DOI：10.11653/syfj2013200103
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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姜艳艳, 张乐, 刘娟, 等. 香橼、佛手中黄酮类成分含量测定及HPLC特征图谱研究[J]. 中国实验方剂学杂志, 2013,19(20):103-107.

JIANG Yan-yan, ZHANG Le, LIU Juan, et al. Determinnaiton of Flavonoids and Study on HPLC Characteristic Spectrum of Citrus Fructus and Citrus Sarcodactylis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 103-107.
姜艳艳, 张乐, 刘娟, 等. 香橼、佛手中黄酮类成分含量测定及HPLC特征图谱研究[J]. 中国实验方剂学杂志, 2013,19(20):103-107. DOI： 10.11653/syfj2013200103.

JIANG Yan-yan, ZHANG Le, LIU Juan, et al. Determinnaiton of Flavonoids and Study on HPLC Characteristic Spectrum of Citrus Fructus and Citrus Sarcodactylis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 103-107. DOI： 10.11653/syfj2013200103.

摘要

目的: 建立香橼、佛手药材中黄酮类成分的含量测定方法及HPLC特征图谱。方法: 采用高效液相色谱法对特征图谱进行研究

并对橙皮苷和柚皮苷进行含量测定

色谱柱为Waters SunFireTM C18(4.6 mm×150 mm

5 μm)

流动相乙腈-0.1%磷酸梯度洗脱

检测波长284 nm

流速1.0 mL·min-1

柱温30℃。结果: 所建立的特征图谱可同时用于含量测定

香橼、佛手黄酮类成分分离度良好

柚皮苷线性范围为0.190 4~1.142 4 μg(r=0.999 5)

平均加样回收率为102.98%(n=6);橙皮苷线性范围为0.173 6~1.215 2 μg(r=0.999 5)

枸橼中橙皮苷平均加样回收率为101.19%(n=6)

佛手中橙皮苷平均回收率为99.11%(n=6)。结论: 所建立的特征图谱及含量测定方法快速、准确、可靠

为香橼、佛手药材的质量控制方法提供科学依据

可用于香橼、佛手质量的科学评价。

Abstract

Objective: To establish an HPLC method for determination and characteristic spectrum of flavonoids in Citrus Fructus and Citri Sarcodactylis Fructus. Method: Chromatographic analysis was carried on the coloumn of Waters SunFireTM C18(4.6 mm×150 mm

5 μm)

the mobile phase was acetonitrile-0.1% phosphoric acid at the flow rate of 1.0 mL·min-1;the detection wavelength was set at 284 nm and the coloumn temperature was 30℃. Result: The flavonoids compounds were base-isolated in HPLC characteristic spectrum. The linear range of hesperidin was 0.173 6-1.215 2 μg(r=0.999 5)

the average recovery was 101.19%(n=6) in Citrus medica

and the average recovery was 99.11%(n=6) in Citri Sarcodactylis Fructus. The linear range of naringin was 0.190 4-1.142 4 μg(r=0.999 5)

the average recovery was 102.98%(n=6). Conclusion: The method is rapid

accurate and reliable

and can be applied to the quality control of Citrus Fructus and Citri Sarcodactylis Fructus.

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