健脾益气活血汤影响血管紧张素l-7相关信号通路调节自发性高血压大鼠血管活性因子释放机制研究

曲怡; 张立德; 王珊珊; 陈志娟; 谷丽艳

doi:10.11653/syfj2013200190

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健脾益气活血汤影响血管紧张素l-7相关信号通路调节自发性高血压大鼠血管活性因子释放机制研究

更新时间：2024-01-04

- 健脾益气活血汤影响血管紧张素l-7相关信号通路调节自发性高血压大鼠血管活性因子释放机制研究
- Effect of Jianpi Yiqi Huoxue Decoction on SHR Releasing Vasoactive Substances by Regulating ACE2-Ang-(l-7)-MAS-AKT Signal Pathways Regulate Releasing Vasoactive Substances
- 中国实验方剂学杂志 2013年19卷第20期页码：190-194
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁省科技厅自然科学基金项目(20102150)
- DOI：10.11653/syfj2013200190
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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曲怡, 张立德, 王珊珊, 等. 健脾益气活血汤影响血管紧张素l-7相关信号通路调节自发性高血压大鼠血管活性因子释放机制研究[J]. 中国实验方剂学杂志, 2013,19(20):190-194.

QU Yi, ZHANG Li-de, WANG Shan-shan, et al. Effect of Jianpi Yiqi Huoxue Decoction on SHR Releasing Vasoactive Substances by Regulating ACE2-Ang-(l-7)-MAS-AKT Signal Pathways Regulate Releasing Vasoactive Substances[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 190-194.
曲怡, 张立德, 王珊珊, 等. 健脾益气活血汤影响血管紧张素l-7相关信号通路调节自发性高血压大鼠血管活性因子释放机制研究[J]. 中国实验方剂学杂志, 2013,19(20):190-194. DOI： 10.11653/syfj2013200190.

QU Yi, ZHANG Li-de, WANG Shan-shan, et al. Effect of Jianpi Yiqi Huoxue Decoction on SHR Releasing Vasoactive Substances by Regulating ACE2-Ang-(l-7)-MAS-AKT Signal Pathways Regulate Releasing Vasoactive Substances[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 190-194. DOI： 10.11653/syfj2013200190.

摘要

目的: 探讨健脾益气活血汤通过血管紧张素转换酶2(ACE2)、血管紧张素1-7(Ang 1-7)-MAS受体、蛋白激酶B(AKT)信号通路调节自发性高血压大鼠(SHR)血管活性因子释放的作用。方法: 将60只24周龄SHR分为SHR组(给予蒸馏水)、培哚普利组(0.4 mg·kg-1·d-1)、培哚普利联合中药组(培哚普利0.4 mg·kg-1·d-1+健脾益气活血汤20 g·kg-1·d-1

中加西组)、健脾益气活血汤高、中、低剂量组(40

10 g·kg-1·d-1)

每组10只

以同龄同种系正常血压的京都种大鼠(WKY)10只作为WKY组(给予蒸馏水)。无创血压计监测大鼠尾动脉收缩压;治疗6周后腹主动脉取血测定血浆Ang(1-7)含量

取肾脏提取总RNA及总蛋白

RT-PCR法检测ACE2

MAS mRNA含量表达

Western blot法检测AKT蛋白表达水平。结果: SHR组ACE2

Ang(1-7)

MAS

AKT含量与WKY组相比明显减少(P<0.01);培哚普利组、中加西组收缩压与SHR组相比明显降低(P<0.01);培哚普利组、中加西组、中药高剂量组ACE2

Ang(1-7)

MAS

AKT含量明显高于SHR组 (P<0.01)。结论: 健脾益气活血汤通过ACE2-Ang (l-7)-MAS-AKT信号通路平衡血管内皮舒缩因子的分泌与释放

发挥改善血管内皮功能及抗高血压作用。

Abstract

Objective: To study the effect of the Chinese medicinethat Jianpi Yiqi Huoxue decoction on angiotensin converting enzyme 2(ACE2)-angiotensin (1-7)[Ang(1-7)]-MAS-protein kinase B(AKT)signal pathways in spontaneously hypertensive rats(SHR). Method: Sixty 24-week-old SHRs were randomly divided into six groups:SHR group (distilled water)

perindopril group (perindopril 0.4 mg·kg-1·d-1)

perindopril plus herb group (perindopril 0.4 mg·kg-1·d-1+10 g·kg-1·d-1of herb)

high

medium and low herb group (40

10 g·kg-1·d-1). And Wistar kyoto rats(WKY) group. The changes of systolic pressure were measured by BP-98A intelligent sphygmomanometer. After six weeks' treatment

the content of Ang(1-7)

ACE2

MAS

AKT was determined. Result: The systolic pressure in the perindopril group and the perindopril plus herb group was prominently decreased

compared with SHR group (P<0.01). The content of Ang(1-7)

ACE2

MAS

AKT in the SHR group was lower than those in the WKY group

the perindopril group

the perindopril plus herb group

and the high dose of herb groups(P<0.01). Conclusion: Jianpi Yiqi Huoxue decoction can promote endothelial vasomotor factors secretion through the ACE2-Ang (l-7)-MAS-AKT signaling pathway.

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