Objective: To observe Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity of esophageal mucosa in reflux esophagitis(RE) rats

and to investigate the fundamental pathogenesis of RE and the therapeutic mechanism in treating RE with Xuanfu Daizhe decoction. Method: Ninty six male Wistar rats were divided into 6 groups:sham operation group

model group

Xuanfu Daizhe decoction high

middle and low dose group (24

12

6 g·kg-1)and western medicine (OME 10 mg·kg-1 plus Mosapride 2 mg·kg-1) group

16 rats each group. Esophagus-duodenum anastomosis was used to establish rat RE model. From 3 days after operation

NS was given to the sham operation group rats and model group rats by gavage feeding;Xuanfu Daizhe decoction and western medicine (OME 10 mg·kg-1 plus mosapride 2 mg·kg-1) was given to the medicine treated groups

and execute all rats in the 8th day

then observe mucous gross change of lower esophagus with eyes and optical microscope. At last

detect the expression of enzymatic activity of Na+-K+-ATP and Ca2+-Mg2+-ATP by chemical methods. Result: Compared with sham operation group

the mortality of the model group was higher;the pathological changes and score were increased;the Na+-K+-ATPase and Ca2+-Mg2+-ATPase of esophageal mucosa were decreased. The mortality of the high and middle dose group of Xuanfu Daizhe decocton treated group was decreased significantly compared with the model group (P<0.05). The rats weight of Xuanfu Daizhe decoction treated group were increased significantly compared with the model group (P<0.05). The pathological changes and score were all reduced in Xuanfu Daizhe decoction high and middle dose group(P<0.01);both Na+-K+-ATPase and Ca2+-Mg2+-ATPase of esophageal mucosa were increased in Xuanfu Daizhe decoction high and middle dose group (P<0.01). Conclusion: Xuanfu Daizhe decoction could increase the activity of Na+-K+-ATPase and Ca2+-Mg2+-ATPase

and protects the cellular integrity of esophageal mucosa

reducing the esophageal mucosal injury of RE.