Objective: Explore the biological mechanisms of the premature ovarian failure in rats by curing with Dingjing decoction. Method: Ninty SD rats were randomly divided into three groups:normal groups

model groups and Dingjing decoction groups;biomolecules which associated with premature ovarian failure:β-endorphin(β-EP)

interleukin-1(IL-1)

nitric oxide synthase(NOS)

nitric oxide synthase(NO)

estrogen receptor(ER)

progesterone receptor(PR)

gonadotropin releasing hormone(GnRH)

corticotropin releasing hormone(CRH)

follicle-stimulating hormone(FSH)

luteinizing hormone(LH)

estradiol(E2)

progesterone(P)

adrenocor ticotropic hormore(ACTH) and cortisol(CORT)were detected. Regulation relations of biological molecules were explored in curing by Dingjing decoction based on factor analysis. And to explore the key biomolecules in premature ovarian failure by curing with Dingjing decoction. Result: The master regulatory factor mainly contains 4 pituitary-ovarian axis biomolecules

pituitary-adrenal axis biomolecule CORT and parts of hypothalamus biomolecules in curing state by Dingjing decoction. And IL-1 is common biomolecules in the hypothalamus part of the curing state's first and second common factor. Conclusion: On the one hand

Dingjing decoction can adjust the pituitary-adrenal axis molecules directly

on the other hand

it can indirectly adjust the pituitary-adrenal axis molecules by hypothalamus. Among the factors

the molecules IL-1 play a pivotal role.