Objective: To optimize processing technology of Strychni Semen with vinegar. Method: Single factor tests and orthogonal test were optimized processing technology of Strychni Semen with vinegar

effects of kinds of vinegar

ratio of drug and liquid

vinegar soaking time and blanching time on the contents of brucine and strychnine were investigated.The contents of brucine and strychnine were determined by HPLC

chromatographic conditions were as follows:Elite Hypersil BDS C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-water-diethylamine(34:100:1)

detection wavelength 258 nm

flow rate 1 mL·min-1

column temperature 30 ℃. Result: The best processing technology of Strychni Semen with vinegar was:balsamic vinegar

proportion of drug and liquid(3:14)

vinegar soaking time 1 d and blanching time1 d.The contents of brucine and strychnine were 0.131 5%

0.177 3%

respectively. Conclusion: Optimized processing technology was stable

feasible and reproducible

it could be used to reduce toxicity of Strychni Semen.