Objective: To investigate effects of temperature

time in processing process of Ginseng Radix et Rhizoma Rubra on the content of ginsenosides. Method: The content of total saponins was determined by UV

orthogonal test was adopted to investigated effects of heating time

steaming temperature

steaming time

drying temperature on total saponins content.The contents of ginsenoside Rb1

Rb2

Rc

Rd

Re

Rg1

Rg2

Rg3

Rh1

Rh2 were proformed by HPLC

chromatographic conditions were as follows:ZORBAX SB-C18 column(2.1 mm×100 mm

3.5 μm)

column temperature 30 ℃

mobile phase of water-acetonitrile gradient elution

flow rate 1.0 mL·min-1

detection wavelength 203 nm

injection volume 5 μL;Effects of different steaming and drying temperature on kinds of ginsenosides were compared by high resolution liquid chromatography-quadrupole-time of flight mass spectrometry. Result: Optimum processing technology for the highest content of total saponins was:temperature raised to 100 ℃ at 60 min

steamed for 6 h and dried at 50 ℃

steaming and drying temperature had significant difference.Under different steaming and drying temperature

the contents of ginsenoside Rb1

Rb2

Rc

Rd

Re

Rg1

Rg2

Rg3

Rh1

Rh2 changed remarkably. Conclusion: According to needs of actual production

processing method of Ginseng Radix et Rhizoma Rubra should adjust for quality control.This study would provide a reference for production and new drugs development of ginsenosides.