Objective: To optimize extraction process of gallic acid in Acalypha australis from Guizhou. Method: The content of gallic acid from A. australis was determined by HPLC

Diamonsil C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-0.05% phosphoric acid(4:96)

detection wavelength 270 nm

column temperature at room temperature

flow rate 1 mL·min-1

injection volume 10 μL.With transfer rate of gallic acid as index

single factor tests were adopted to screen extraction methods and solvents

effects of ratio of solid-liquid

ethanol concentration

extraction temperature and time on extraction technology were investigated by orthogonal test. Result: The best extraction process of gallic acid was as following:extracted 2 times with 30 times the amount of 70% ethanol at 90 ℃

4 hours per time.Average transfer rate of gallic acid was 7.888 mg·g-1(RSD 1.60%). Conclusion: This optimized extraction process was stable and feasible

it could provide experimental basis for development and utilization of A. australis from Guizhou.