Objective: To investigate effects of high pressure processing on contents of emodin

physcion and 2

3

5

4'-stilbene glucoside from Polygoni Multiflori Radix. Method: Polygoni Multiflori Radix was processed by high pressure steaming method

effects of different steaming time on contents of anthraquinones and 2

3

5

4'-stilbene glucoside were investigated

and properties change during processing was observed.The contents of emodin and physcion were determined by HPLC

chromatographic conditions were as follows:Diamonsil C18 column(4.6 mm×250 mm

5 μm)

mobile phase of methanol-0.1% phosphoric acid(80:20)

flow rate 1.0 mL·min-1

detection wavelength 254 nm

column temperature 30 ℃

injection volume 10 μL.HPLC conditions of 2

3

5

4'-stilbene glucoside were as follows:mobile phase of acetonitrile-water gradient elution

detection wavelength 320 nm;other conditions were the same as these above conditions. Result: The content of 2

3

5

4'-stilbene glucoside were 4.55%

3.54%

2.20%

1.87%

1.52%

1.15%

0.76% after high pressure steamed 0

1

2

3

4

5

6 h.By increasing of steaming time

contents of incorporating anthraquinones and 2

3

5

4'-stilbene glucoside from Polygoni Multiflori Radix gradually decreased

but the content of free anthraquinones increased. Conclusion: As tonics applications

Polygoni Multiflori Radix should be heated for four hours under high pressure.