Objective: To optimize prescription of total saponins from leaves of Notoginseng Radix et Rhizoma liposomal gels and investigate its in vitro transdermal absorption properties. Method: Total saponins from leaves of Notoginseng Radix et Rhizoma liposomes were prepared by thin-film dispersion Method

with encapsulation efficiency as index

orthogonal test was adopted to optimize liposomes formulation by taking ratio of phospholipids-cholesterol

lipids-drug ratio and drug concentration as factors.High-speed centrifugation method was used to determine encapsulation efficiency

carbomer 940 was added as a substrate for preparation of gels

its dosage was investigated by single factor test.In vitro transdermal test was adopted to investigate in vitro transdermal absorption of ginsenoside Rb3 from total saponins in leaves of Notoginseng Radix et Rhizoma

the content of ginsenoside Rb3 was determined by HPLC

chromatographic conditions were as follows:ZORBOX SB-C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile(A)-0.2% phosphoric acid(B) gradient elution(0-19 min

30% A;19-21 min

35% A;21-26 min

50% A)

detection wavelength 203 nm. Result: Optimized formulation technology was:lecithin-cholesterol(4:1)

the concentration of ginsenoside Rb3 90 g·L-1

lipid-drugs(6:1)

the highest cumulative permeation rate of drug through skin when the content of carbomer 940 was 0.5%. Conclusion: Liposomal gels could increase permeation amount of active ingredients in skin

this study laid experimental foundation for development of new dosage forms and new administration routes.