Objective: To study the effect of the processing methods of the heating and the steaming on the content of six ester-type alkaloids in Radix Aconite Lateralis. Method: By using the processing methods of the high temperature-heating and the high pressure-steaming to control the temperature and time

six ester-type alkaloids in the different processed products of Radix Aconite Lateralis were determined. Result: There is a significant difference on the content of six ester-type alkaloids between the crude and the processed Radix Aconite Lateralis. It is significantly reduced or disappeared on the content of diester-type alkaloids and increased on monoester-type alkaloids. The linear ranges of benzoylmesaconine

benzoylaconine

benzoylhypaconine

mesaconitine

hypaconitine and aconitine were 0.032 184-3.218 4 μg (r=0.999 9)

0.030 016-3.001 6 μg (r=0.999 9)

0.031 320-3.132 0 μg (r=0.999 9)

0.030 744-3.074 4 μg (r=0.999 9)

0.030 912-3.091 2 μg (r=0.999 9)

0.031 920-3.192 0 μg (r=0.999 9) and the average recoveries were 100.03%

99.99%

98.16%

100.68%

99.27%

102.81% and RSDs were 1.35%

1.96%

1.01%

1.03%

0.55%

0.91% respectively. Conclusion: These processing methods are simple and controllable and can provide new references on processing radix aconite lateralis.