Objective: To establish a quality control method of Feire Qingjie oral liquid with chlorogenic acid as index. Method: Mori Cortex and Phragmitis Rhizoma from Feire Qingjie oral liquid were identified qualitatively by TLC.HPLC was adopted to determine the content of chlorogenic acid from Feire Qingjie oral liquid

chromatographic conditions included Haito Pack ODS column(4.6 mm×250 mm

5 μm)

methanol-1% glacial acetic acid(1:4) as mobile phase

detection wavelength at 326 nm

flow rate 1.0 mL·min-1

injection volume 10 μL

column temperature at 30 ℃. Result: By comparing with reference drug chromatography

Mori Cortex and Phragmitis Rhizoma showed the same spots on corresponding position without interference of negative control.Chlorogenic acid showed a good linear relationship in the rang of 3.180-106.0 mg·L-1 with r=1

average recovery was 100.5% with RSD 2.05%

the average mass concentration of chlorogenic acid from four batches of samples were 28.9

42.5

28.6

21.7 mg·L-1. Conclusion: This established method was convenient

quick

accurate and available for one of quality control methods for Feire Qingjie oral liquid.