Objective: To establish the method for determining curdione

curcumol

germacrone

curzerene

furanodiene and β-elemene in Curcuma zedoary by RP-HPLC. Method: A Hypersil ODS column (4.6 mm×250 mm

5 μm) was used with the mixture of acetonitrile-0.1% phosphoric acid as the mobile phase in gradient elution. The flow rate was 1.0 mL·min-1. The column temperature was kept at 25 ℃. The detection wavelength was set at 215 nm. Result: The calibration curves of curdione

curcumol

germacrone

curzerene

furanodiene and β-elemene were in good linearity over the ranges of 0.544-5.44 μg (r=0.999 6)

0.414-4.14 μg (r=0.999 8)

0.122-1.22 μg (r=0.999 5)

1.78-17.8 μg (r=0.999 3)

0.318-3.18 μg (r=0.999 4)

0.506-5.06 μg (r=0.999 5) respectively

and the average recoveries were 99.60% (RSD 2.71%)

101.48% (RSD 1.37%)

99.50% (RSD 2.47%)

100.29% (RSD 2.52%)

99.87% (RSD 1.51%)

100.58% (RSD 1.33%) respectively. Conclusion: The method is sensitive

accurate;the separation effect is good without interference. It can provide a basis as evaluation standard for quality of Rhizoma Zedoariae

and provide scientific basis for clinical rational drug use.