Objective: To investigate the effect of Erigerontis Herba and Paeoniae Radix Rubra compound with different proportion and two different routes of administration(intravenous and oral) on brain damage in the rat model of cerebral focal ischemia and reperfusion. Method: SD rats were divided into four groups randomly

normal control group

ischemia-reperfusion group

positive control group and different proportion of Erigerontis Herba and Paeoniae Radix Rubra compound group

16 in each group. The Longa method was used to establish the MCAO model. Paeoniae Radix Rubra compound at different proportion was injected or given by gavage at different time points according to the test design. Based on factorial experiment design and factor analysis method

the whole combinations of each factors(route

proportion) and each levels(four different ratio of formula) were designed. The influence significance of each factors and their interaction for indexes were analyzed. Also

the significant difference of all the levels of every factor was observed. Result: The compatibility of Erigerontis Herba and Paeoniae Radix Rubra at a ratio of 1:1.5 was found

which could obviously improve pivotal biological indicator

and include neuroethology

the rate of cerebral infraction

and so on. Conclusion: The compatibility ratio of Erigerontis Herba and Paeoniae Radix Rubra of is 1:1.5

which exerts a protective effect on cerebral ischemia-reperfusion injury.