镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氢酸羟化酶表达的影响

兴桂华; 王晓丽; 丛欢; 邹宇; 李丽波; 董妙先

doi:10.11653/syfj2013210190

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镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氢酸羟化酶表达的影响

更新时间：2024-01-04

- 镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氢酸羟化酶表达的影响
- Effect of Zhengan Xifeng Decoction on Expression of 14-3-3 and Tyrosine Hydroxylase Gene in Substantia Nigra of MPTP-treated C57BL/6 Mice
- 中国实验方剂学杂志 2013年19卷第21期页码：190-193
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81241107)
- DOI：10.11653/syfj2013210190
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- 纸质出版日期：2013
- 稿件说明：
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兴桂华, 王晓丽, 丛欢, 等. 镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氢酸羟化酶表达的影响[J]. 中国实验方剂学杂志, 2013,19(21):190-193.

XING Gui-hua, WANG Xiao-li, CONG Huan, et al. Effect of Zhengan Xifeng Decoction on Expression of 14-3-3 and Tyrosine Hydroxylase Gene in Substantia Nigra of MPTP-treated C57BL/6 Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 190-193.
兴桂华, 王晓丽, 丛欢, 等. 镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氢酸羟化酶表达的影响[J]. 中国实验方剂学杂志, 2013,19(21):190-193. DOI： 10.11653/syfj2013210190.

XING Gui-hua, WANG Xiao-li, CONG Huan, et al. Effect of Zhengan Xifeng Decoction on Expression of 14-3-3 and Tyrosine Hydroxylase Gene in Substantia Nigra of MPTP-treated C57BL/6 Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 190-193. DOI： 10.11653/syfj2013210190.

摘要

目的: 观察镇肝熄风汤对1-甲基-4-苯基-1

6-四氢吡啶(1-methy-4-phenyl-1

6-tetrahy-dropyridine MPTP)诱导的C57BL/6小鼠帕金森病模型自主活动的影响及其可能机制。方法: 连续灌胃14 d给予不同剂量的镇肝熄风汤(12.5

50 g·kg-1)和司来吉兰(10 mg·kg-1)预处理

于给药第11天开始连续4 d C57BL/6小鼠皮下注射MPTP(30 mg·kg-1)制备帕金森病小鼠模型。通过自主活动实验观察动物行为学表现

采用实时定量RT-PCR法检测多功能蛋白14-3-3和酪氨酸羟化酶(tyrosine hydroxylase

TH) mRNA表达

Western blot方法检测多功能蛋白14-3-3

TH蛋白表达。结果: 与模型组比较

镇肝熄风汤可显著增强帕金森病小鼠自主活动能力(均P<0.05)

上调多功能蛋白14-3-3和TH mRNA和蛋白表达(均P<0.05)。结论: 镇肝熄风汤具有抗实验性帕金森病的作用

其机制可能与上调多功能蛋白14-3-3和TH的mRNA和蛋白表达有关。

Abstract

Objective: The aim of this study was to investigate the effects of Zhengan Xifeng decoction on the spontaneous movement in 1-methy-4-phenyl-1

6-tetrahy-dropyridine(MPTP)-treated C57BL/6 mice

and some mechanisms involved. Method: C57BL/6 mice were given MPTP (30 mg·kg-1) to prepare Parkinson disease mice model. Different doses of Zhengan Xifeng decoction (12.5

50 g·kg-1)

or selegiline (10 mg·kg-1) were given for 14 d in the pretreatment groups. The behavioral analysis of C57BL/6 mice was performed by using spontaneous movement test. Real-time PCR and Western blot were used to analyze the expression of 14-3-3 and tyrosine hydroxylase (TH) mRNA and protein. Result: Zhengan Xifeng decoction could reduce the number of spontaneous movement of MPTP-treated C57BL/6 mice. Zhengan Xifeng decoction could also up-regulate the expression of 14-3-3 and TH mRNA and protein. Conclusion: Anti-Parkinson disease effect of Zhengan Xifeng decoction may be attributable to up-regulation mRNA and protein expression of 14-3-3 and TH in MPTP-treated C57BL/6 mice.

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