ZHAO Lei, HUANG Shan, JIN Yan, et al. Antioxidant Effect of Each Part of Ethanol Extrac of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 210-213.
ZHAO Lei, HUANG Shan, JIN Yan, et al. Antioxidant Effect of Each Part of Ethanol Extrac of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 210-213. DOI: 10.11653/syfj2013210210.
Objective: To study the scavenging activity in vitro of each part of ethanol extract of Rhamnella gilgitica Mansfeild et Melch. Method: R. gilgitica was extracted with 70% ethanol
then was extracted by petroleum ether
dichloromethane
ethyl acetate
n-butyl alcohol
ethanol the each part was configured to the appropriate solution
Prussian blue method Fenton method and 2
2-diphenyl-1-picrylhydrozyl(DPPH) were used to measure the antioxidantactivity. Result: The ethyl acetate
n-butyl alcohol and ethanol extract of R. gilgitica showed obvious scavenging effects
IC50 of the ethyl acetate
n-butyl alcohol and ethanol extract of R.gilgitica with DPPH was 0.060 5
0.076 3
0.083 3 g·L-1
respectively. Conclusion: The scavenging activity showed a dose-response relationship
even reach 60% by the above 1.0 g·L-1concentrations.The high polarity of solvents layer extract has the strongest free radical scavenging activity.