Objective: To study the potent active components of Cimicifugae Rhizoma for treating hepatitis B virus(HBV). Method: The total phenolic acid and saponins of Cimicifugae Rhizoma were separated by macroporous resin

HBV DNA

HBsAg and HBeAg were observed to analyze the anti-hepatitis B virus effect of prim-o-glucosylcimifugin

ferulic acid

Cimicifugae Rhizoma aqueous extract solution

Cimicifugae Rhizoma phenolic acid and Cimicifugae Rhizoma saponins. Result: The five groups significantly reduced the concentration of HBsAg and HBeAg. Compared with the blank control group

the total phenolic acid can decrease the HBsAg and HBeAg (P<0.01) and the ferulic acid was the potential active component

Cimicifugae Rhizoma saponins can reduce the HBsAg (P<0.05) but prim-o-glucosylcimifugin have no obvious anti-hepatitis B virus effect. The total phenolic acid of Cimicifugae Rhizoma can remarkably decrease the level of viral core associated-HBV DNA in the cytoplasm. Conclusion: The total phenolic acid of Cimicifugae Rhizoma is the active part to anti-hepatitis B virus effect

and the results provide the basis of screening new drugs for treatment hepatitis B virus.