Objective: To observe the analgesic effect of Wumei pill and its disassembled prescriptions on ulcerative colitis (UC) in rats. Method: Experimental animals were divided into control group

model group

Wumei pill group

cold and heat group

heat group

cold group

tonic group

convergence group. According to the decomposition principle

Each group was respectively given water decoction of Wumei pill(13.3 g·kg-1)

cold and heat prescription(9.0 g·kg-1)

heat prescription (5.33 g·kg-1)

cold prescription (3.67 g·kg-1)

convergence prescription(2.67 g·kg-1)

and tonic prescription(1.67 g·kg-1) by gavage. The control group and model group were given with saline(10 mL·kg-1). Analgesic effect of Wumei pill and its prescriptions were observed by hot plate and writhing. Ulcerative colitis model was established with trinitro-benzene-sulfonic acid(TNBS)/ethanol. The PGE2 content of colonic mucosa was determined by enzyme-linked immunosorbent assay. Result: Compared with blank group

the Wumei pill group

cold and heat group and heat group could prolong the pain threshold of mice

reduce the writhing times and decrease the PGE2 content of colonic mucosa in rats(P<0.05). Tonic group had partly analgesic effect but the effect was weaker than Wumei pill group(P<0.05). Conclusion: Wumei pill has obvious analgesic effect

while tonic group has partly analgesic effect. The drug of heat group play an very important role for analgesic effect.