Objective: Investigation on RP-HPLC-ELSD fingerprint of triterpenes in Poria cocos from different origin place

in order to provide a theory evidence to evaluate the quality of P. cocos from different origin place

and improve the quality of P. cocos. Method: The chromatographic conditions were Kromasil 100-5 C18 column (4.6 mm×250 mm

5 μm)

0.2% methanoic acid (A) and acetonitrile (B) as gradient mobile phases

flow rate being 1.0 mL·min-1

column temperature at 30 ℃

evaporative light-detection conditions:atomization temperature at 40 ℃

pressure of 350 kPa

gain value of 7

and 20 μL sample injection. Result: Fingerprint was established with 18 common peaks and methodology reached the required standard. Conclusion: The method is simple

rapid

accurate

and can be used as an effective means of P. cocos quality evaluation of different origin place.