Objective: To optimize extraction technology of Qingzao Jiufei decoction by multi-index comprehensive evaluation method. Method: L9(34) orthogonal design was adopted

with dry extract rate

the contents of amygdalin

chlorogenic acid and glycyrrhizic acid as comprehensive evaluation index

effects of ethanol concentration

decocting time and times on extraction technology were investigated by composite score method.The contents of three index ingredients were determined by HPLC

chromatographic conditions were as follows:Agilent TC-C18 column(4.6 mm×250 mm

5 μm)

detection wavelength program(0-50 min

207 nm;50-100 min

237 nm)

column temperature 30 ℃

flow rate 1.0 mL·min-1

mobile phase of acetonitrile(A)-0.1% phosphoric acid(B) gradient elution(0-30 min

5%-10%A;30-55 min

10%-20%A;55-80 min

20%-35%A). Result: Optimal extraction technology was as following:extracted 2 times with 8 times the amount of 70% ethanol for 2 h per time. Conclusion: This optimized extraction technology was stable

feasible and reasonable with high extraction efficiency of active ingredients.