Objective: To evaluate anti-platelet aggregation of 6

7-dehydroroyleanone in roots of Salvia deserta and optimize its extraction process. Method: In vitro anti-platelet aggregation of 6

7-dehydroroyleanone was determined by turbidimetry.Based on single factor tests

orthogonal test was adopted to effects of extracting times

solid-liquid ratio

extracting time on extraction technology of 6

7-dehydroroyleanone with yield of 6

7-dehydroroyleanone as index.The content of 6

7-dehydroroyleanone was determined by HPLC with mobile phase of methanol-0.02% formic acid(83: 17)

detection wavelength of 272 nm

column temperature at 40 ℃. Result: By comparing with the saline group

the high and middle dose group of 6

7-dehydroroyleanone could effectively inhibit platelet aggregation induced by thrombin

platelet aggregation inhibition rates of the high dose group of 6

7-dehydroroyleanone within 15

30

45

60 min were 90%

75%

66%

64%

respectively.Optimum extraction process was as following:extracted 3 times with ten-fold the amount of 95% ethanol at 85 ℃ for 3 h each time;The content of 6

7-dehydroroyleanone in roots of S. deserta was 0.01%. Conclusion: 6

7-Dehydroroyleanone in roots of S. deserta showed better anti-platelet aggregation effect

optimized extraction technology was stable

reasonable and feasible which based on the activity.