Objective: To investigate pretreatment and purification processes of total flavonoids from Scutellariae Barbatae Herba with AB-8 macroporous adsorption resin. Method: Pretreatment process of total flavonoids extract from Scutellariae Barbatae Herba was investigated with transfer rate of scutellarin

clarity and solution color as indexes.With transfer rate of scutellarin and dry extract yield as indexes

single factor tests were adopted to optimize purification technology of total flavonoids from Scutellariae Barbatae Herba with AB-8 macroporous resin. Result: Transfer rate of scutellarin was up to 90% after pretreated by adjusting pH of extract solution to 3.0-3.3 and centrifuging two times.Optimum purification process parameters of total flavonoids with AB-8 resin were as follows:diameter-height ratio 1: 5

the concentration of sample solution 0.17 g·mL-1

sample volume (equivalent as crude drug)-resin volume ratio 1: 2

sampling flow rate 2 BV·h-1

elution volume with water 4.5 BV

elution solvent 40% ethanol

elution volume with 40% ethanol 5.5 BV

elution velocity 4 BV·h-1;Under these conditions

transferring rate of scutellarin was about 93% with the mass fraction of 8%

dry extract yield was 2.5%. Conclusion: Extract yield and color was significantly reduced after pretreated extraction solution of total flavonoids

and optimized purification process was reasonable

feasible and suitable for industrial production of total flavonoids from Scutellariae Barbatae Herba.