Objective: To optimize ethanol extraction technology of Yinchen Siling granules. Method: With yield of geniposide as index

single factor test was adopted to screen ethanol extraction method

such as refluxing method

ultrasonic method and percolation method;Effects of ethanol concentration

soaking time

ethanol amount and percolation velocity on extraction technology was investigated by orthogonal design.The content of geniposide was determined by HPLC

chromatographic conditions were as follows:UltimateTMC18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-water(15: 85)

flow rate 1 mL·min-1

column temperature 40 ℃

detection wavelength 238 nm

injection volume 10 μL. Result: Ethanol percolation method was adopted

the best extraction conditions were as follows:soaked 12 h by adding 10 times the amount of 70% ethanol with percolation velocity of 5 mL·min-1;Under these conditions

yield of geniposide was up to 42.524 4 mg·g-1. Conclusion: This optimized extraction process was stable

reasonable and practical

it could provide a theoretical basis for industrial production of Yinchen Siling granules.