Objective: To optimize percolation process of Strychni Semen from Yaotongning plasters. Method: With transferring rate of strychnine as index

orthogonal test was adopted to optimize percolation extraction technology with the concentration and amount of ethanol

percolation rate as factors.The content of strychnine was determined by HPLC

chromatographic conditions were as follows:mobile phase of methanol-water-acetic acid(20: 96: 7

pH was adjusted to 3.10 with triethylamine)

flow rate 1.0 mL·min-1

detection wavelength 254 nm. Result: Optimum percolation process was added 15 times the amount of 70% ethanol with percolation speed of 2 mL·kg-1·min-1.Under these conditions

transferring rate of strychnine was more than 90%. Conclusion: Optimized extraction technology was reasonable

convenient and feasible

this study had guiding significance for industrial production of Yaotongning plasters.