Objective: To optimize extraction technology of compound Biqing tablets. Method: Based on single factor tests

orthogonal design was adopted to optimize ethanol extraction technology of Smilacis Chinae Rhizoma and other six herbs with composite score of diosgenin content

colchicine content and extract yield as index;With extract yield and the content of total polyphenols as comprehensive evaluation index

water extraction technology of Phyllanthi Fructus and other five herbs was optimized by orthogonal test.The contents of diosgenin and colchicine were determined by HPLC

chromatographic conditions were as follows:Cosmosil C18 column(4.6 mm×250 mm

5 μm)

column temperature 35 ℃

flow rate 1.0 mL·min-1

injection volume 10 μL

detection wavelength at 203

350 nm

mobile phase with methanol-water(90: 10) and methanol-water(37: 63);The content of total polyphenols was determined by UV. Result: Optimum ethanol extraction technology was as following:extracted 2 times with 10 times the amount of 70% ethanol

1 h per time;Optimum water extraction technology was:extracted 3 times with 10 times the amount of water

1 h each time. Conclusion: This optimized technology was stable and feasible

it could provide an experimental basis for quality control of compound Biqing tablets.