Objective: To establish HPLC characteristic fingerprint of Jiagasong decoction. Method: Taking 6-gingerol and dehydrodiisoeugenol as control components

which were derermined by HPLC

chromatographic conditions were as follows:Platisil ODS column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-methanol-0.3% phosphoric acid solution for gradient elution

detection wavelength 274 nm

column temperature 25 ℃

flow rate 1.0 mL·min-1

injection volume 10 μL.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004A) was used in analysis 10 batches of Jiagasong decoction. Result: HPLC characteristic fingerprint of Jiagasong decoction was established

15 common peaks were received

two of them were identified as 6-gingerol and dehydrodiisoeugenol.Similarity of fingerprint among 10 batches of samples was more than 0.9

indicating a good correlation and basically the same chemical compositions. Conclusion: This assay method was simple

sensitive and accurate

this paper could provide scientific basis for quality evaluation of Jiagasong decoction.