Objective: To develop the qualitative and quantitative methods for quality control of Mushu pills. Method: TLC were used as qualitative identifications of Paeoniae Radix Alba and Corydalis Rhizoma in Mushu pills;And to develop a HPLC method for determination of paeoniflorin in Mushu pills

separation was performed on ZORBAX SB-C18 column(4.6 mm×150 mm

5μm)with acetonitrile-0.1% phosphoric acid(15: 85) as mobile phase

flow rate of 1.0 mL·min-1

detection wavelength was at 230 nm

injection volume 10 μL

column temperature was 30 ℃. Result: TLC characteristic identification was distinct and highly specific.Paeoniflorin showed a good liner relationship within the range of 37.80-756.00 ng

the average recovery was 98.2% with RSD 1.4%. Conclusion: This established determination method was reliable

accurate and specific

it could be used for quality control of Mushu pills.