Objective: To investigated effects of different harvesting times and different processing methods on the contents of caffeic acid and rosmarinic acid from Isodon lophanthoides. Method: The contents of caffeic acid and rosmarinic acid were determined by HPLC

which was performed on a Dikma Diamonsil C18(2) column(4.6 mm×250 mm

5 μm)

gradient elution(0-17 min

12%A;17-20 min

12%-25%A;20-50 min

25%A) with mobile phase of acetonitrile-0.3% phosphoric acid

flow rate was 1.0 mL·min-1

column temperature was room temperature

detection wavelength was 329 nm

injection volume 20 μL.With the contents of caffeic acid and rosmarinic acid as indexes

effects of harvesting times(May to November) and processing methods(drying in shade

sun-dried

drying) on quality of I. lophanthoides were investigated. Result: Different harvesting times had little effect on the content of caffeic acid from stems and leaves of I. lophanthoides

but it had significant effect on the content of rosmarinic acid.After handled by three drying methods

yield of rosmarinic acid were 1.365%

1.790%

1.577%

respectively;Yield of caffeic acid were 0.057%

0.053%

0.055%

respectively. Conclusion: I. Lophanthoides harvested in July had the best quality

herbs could be harvested twice a year

harvesting times were in July and November;Processing method of naturally dried was optimum.