Objective: To study the chemical constituents from the seeds of Momordica charantia. Method: The compounds were separated and purified from the title by using a various ch-romatographic techniques including colum chromatography over silica gel

ODS and preparative HPLC.Their structures were elucidated by spectroscopic techniques including 1H-NMR

13 C-NMR

2D-NMR

ESI-MS and HRESI-MS. Result: Ten compounds were isolated from this plant and their structures were identified as 24β-ethyl-5α-cholest-7

anti-22E

25(27)-triolefin-3β-hyd-roxy-3-O-β-D-glucopyranoside(1)

1-methyl-1

2

3

4-tetrahydro-β-carboline-3-carboxylic acid (MT CA)(2)

nicotinamide(3)

uracil(4)

(6S

7E

9S)-6

9

10-trihydroxy-4

7-megastigmadien-3-one (5)

epipinoresinol-4

4'-di-O-D-glucopyranoside(6)

lectin(7)

(6R

9S)roseoside(8)

vicine(9)

6-(2

3-dihydroxyl-4-hydromethyl-tetrahydro-furan-1-yl)-cyclopentene[c]pyrrole-1

3-diol(10). Conclusion: The compounds 2

3

5

6

7

9

10 were isolated from the seed of M. charantia for the first time.