Objective: To study the chemical constituents of the badger oil for the foundation of the quality standards of the badger oil. Method: The compounds were isolated and purified by silica gel column chromatography and preparative layer chromatography methods

and their structures were identified by spectral analysis. HPLC fingerprints of Badger oil and pig oil were compared with the chromatographic conditions as follows:The Phenomenex Hyper C18with cetonitrile-dichloromethanol (75: 25) as the mobile phase was used. The column temperature was 30 ℃

the flow rate was 1.0 mL·min-1 and ELSD was used

the tube temperature was 70 ℃

the press of nitrogen was 3.5 bar. Result: Two compounds were isolated and identified as glycerol trioleate (1) and glycerol linoleate dioleate (2). HPLC fingerprint showed there is big difference in chemical constituents exits between the badger oil and the pig oil. Conclusion: Compounds 1

2 were obtained for the first time from badger oil.