Objective: To establish methods for identification and determination of caffeic acid and rosmarinic acid in Rabdosia. lophanthoides var gerardiana by TLC and HPLC. Method: The developing solvent for caffeic acid and rosmarinic acid in R. lophanthoides var gerardiana by TLC was n-hexane-ethylestate-formic acid (3: 3: 1)

detected under the UV light (365 nm). HPLC method was performed on a Dikma Diamonsil C18 column (4.6 mm×250 mm

5 μm) with a mobile phase of methanol-0.3% phosphoric acid by gradient elution. The flow rate was 1.0 mL·min-1. The column temperature was kept at 25 ℃ and detection wavelength was set at 329 nm. Result: Caffeic acid and rosmarinic acid could be detected with clear spots and good separated by TLC

caffeic acid and rosmarinic acid showed good linearity in the ranges of 0.045 4-0.908 0 μg (r=0.999 9) and 0.219 2-4.384 0 μg (r=0.999 6) with average recoveries of 96.57% (RSD 1.32%) and 99.48% (RSD 2.53%). Conclusion: The methods are accurate and can be used for the quality of R. lophanthoides var gerardiana.