Objective: To investigate the volatile constituents from of Vitex negundo flowers from different regions. Method: The essential oil of V. negundo flowers Jiyuan in Henan province and Dabieshan mountain in Hubei province were analyzed by SPME-GC-MS for the first time. Result: Seventy compounds were isolated from volatiles in two different areas and forty-three were identified. Fourteen compounds were owned in both samples. β-Caryophyllene was the highest content accounted for 40.36% and 32.42% in both samples respectively

followed by cineole and (Z)-b-farnesene in mutual compounds. Conclusion: The results showed that the major components of volatiles from V. negundo flowers in different region were in closer

but the kinds and contents were different.