Objective: To establish a fingerprint of Fufang Tujingpiding by HPLC. Method: The experiment was conducted on Agilent Zorbax SB C18(4.6 mm×250 mm

5 μm)

eluted with methanol (B)-0.09% formic acid aqueous solution (A) as the gradient elution mobile phases. The detective wavelength was set at 260 nm with the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30 ℃. The data was analyzed by Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine software to compare the quality of 14 batches of Fufang Tujingpiding. Result: The standard chromatographic fingerprints of Fufang Tujingpiding was established

13 co-possessing peaks were marked according to the fingerprints of 14 batches of it

and the similarity was greater than 0.96. Conclusion: The established method has good precision

reproducibility and stability

which provides the basis for the quality control of Fufang Tujingpiding as well.