HPLC同时测定京大戟生品和炮制品中3种三萜类成分含量

曾颜; 侯朋艺; 马冰洁; 周增慧; 毕开顺; 陈晓辉

doi:10.11653/syfj2013220141

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HPLC同时测定京大戟生品和炮制品中3种三萜类成分含量

更新时间：2024-01-04

- HPLC同时测定京大戟生品和炮制品中3种三萜类成分含量
- HPLC Simultaneous Determination of Three Triterpenes in Euphorbiae Pekinensis Radix and its Processed Products
- 中国实验方剂学杂志 2013年19卷第22期页码：141-144
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81373367)
- DOI：10.11653/syfj2013220141
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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曾颜, 侯朋艺, 马冰洁, 等. HPLC同时测定京大戟生品和炮制品中3种三萜类成分含量[J]. 中国实验方剂学杂志, 2013,19(22):141-144.

ZENG Yan, HOU Peng-yi, MA Bing-jie, et al. HPLC Simultaneous Determination of Three Triterpenes in Euphorbiae Pekinensis Radix and its Processed Products[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 141-144.
曾颜, 侯朋艺, 马冰洁, 等. HPLC同时测定京大戟生品和炮制品中3种三萜类成分含量[J]. 中国实验方剂学杂志, 2013,19(22):141-144. DOI： 10.11653/syfj2013220141.

ZENG Yan, HOU Peng-yi, MA Bing-jie, et al. HPLC Simultaneous Determination of Three Triterpenes in Euphorbiae Pekinensis Radix and its Processed Products[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 141-144. DOI： 10.11653/syfj2013220141.

摘要

目的: 建立同时测定京大戟生品和炮制品中甘遂甾醇、大戟二烯醇和24-亚甲基环阿尔廷醇含量的方法。方法: 采用HPLC

Agilent XDB C8色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈-水(90:10)

流速1.0 mL·min-1

柱温35 ℃

检测波长205 nm。结果: 甘遂甾醇、大戟二烯醇、24-亚甲基环阿尔廷醇分别在0.506~5.06 μg (r=1.000 0)

1.052~10.52 μg (r=0.999 9)和1.124~11.24 μg(r=0.999 8)线性关系良好。平均加样回收率分别为99.7%(RSD 0.9%)

99.4%(RSD 1.0%)

99.4%(RSD 1.3%)。结论: 方法简便、快速、灵敏度高、专属性好

为京大戟药材及其炮制品质量控制提供依据。

Abstract

Objective: To establish a method for the determination of tirucallol

euphol and 24-methylene cycloartanol in Euphorbiae Pekinensis Radix and its processed products. Method: An HPLC method had been developed for detecting. The separation was performed on an Agilent XDB C8 column (4.6 mm×150 mm

5 μm) at 35 ℃ with acetonitrile-H2O (90: 10) as the mobile phase. The flow rate was 1.0 mL·min-1

and the detection wavelength was set at 205 nm. Result: The linear response range was 0.506-5.06 μg (r=1.000 0) for tirucallol

1.05-10.5 μg (r=0.999 9) for euphol and 1.12-11.2 μg (r=0.999 8) for 24-methylene cycloartanol. The average recoveries of these components were 99.7% (RSD 0.9%)

99.4% (RSD 1.0%) and 99.4% (RSD 1.3%) respectively. Conclusion: The method is simple

accurate and reproducible for quality control of Euphorbiae Pekinensis Radix and its processed products.

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