藤茶的HPLC指纹图谱及化学模式识别研究

苏素娇; 李娜; 陈亮; 林培玲; 丁春花; 陈诗强; 梁一池

doi:10.11653/syfj2013220144

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藤茶的HPLC指纹图谱及化学模式识别研究

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- 藤茶的HPLC指纹图谱及化学模式识别研究
- HPLC Fingerprint and Chemical Pattern Recognition Method of
- 中国实验方剂学杂志 2013年19卷第22期页码：144-147
- 作者机构：
- 作者简介：
- 基金信息：
  
  福建省科技厅社发处重大项目(2011Y4005)
- DOI：10.11653/syfj2013220144
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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苏素娇, 李娜, 陈亮, 等. 藤茶的HPLC指纹图谱及化学模式识别研究[J]. 中国实验方剂学杂志, 2013,19(22):144-147.

SU Su-jiao, LI Na, Chen Liang, et al. HPLC Fingerprint and Chemical Pattern Recognition Method of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 144-147.
苏素娇, 李娜, 陈亮, 等. 藤茶的HPLC指纹图谱及化学模式识别研究[J]. 中国实验方剂学杂志, 2013,19(22):144-147. DOI： 10.11653/syfj2013220144.

SU Su-jiao, LI Na, Chen Liang, et al. HPLC Fingerprint and Chemical Pattern Recognition Method of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 144-147. DOI： 10.11653/syfj2013220144.

摘要

目的: 建立藤茶药材HPLC指纹图谱

并进行主成分聚类分析

为其质量控制提供依据。方法: 采用UltimateXB-C18色谱柱(4.6 mm×250 mm

5 μm);流动相乙腈-0.5%甲酸溶液梯度洗脱

检测波长365 nm;柱温40 ℃;应用SPSS软件对数据进行统计学分析。结果: 建立了藤茶药材HPLC指纹图谱共有模式

确定了11个共有峰

并指认了2个主要共有峰。通过主成分聚类分析

18个藤茶不同无性系种质可分为3类。结论: 该方法快速

可较全面的反映藤茶药材中化学成分的信息

为评价藤茶药材提供了科学实验依据。

Abstract

Objective: To establish an HPLC fingerprint of Ampelopsis grossedentata

and to make evaluation by principal component and cluster analysis for its quality control. Method: The HPLC analysis was performed on a UltimateXB-C18 column (4.6 mm×250 mm

5 μm) with column temperature at 40 ℃. The mobile phase was consisted of water containing 0.5% formic acid and acetonitrile in gradient mode. The detection wavelength was set at 365 nm. All data were analyzed with the SPSS statistical software. Result: The mutual mode of HPLC fingerprint has been established. There were 11 common peaks

two of which were identified. 18 samples from different clonal germplasm were classified into three groups based on principal component and cluster analysis. Conclusion: The method was time-saving and represent the chemical information and provide a scientific basis for quality control of Ampelopsis grossedentata.

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