HPLC测定小活络丸(浓缩丸)中11-羰基--乙酰乳香酸

闵春艳; 付妍; 张亚双; 顾炳仁

doi:10.11653/syfj2013220151

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HPLC测定小活络丸(浓缩丸)中11-羰基--乙酰乳香酸

更新时间：2024-01-04

- HPLC测定小活络丸(浓缩丸)中11-羰基--乙酰乳香酸
- Determination of Acetyl-11-keto--boswellic Acid in Xiaohuoluowan (Concentrated Pills) by HPLC
- 中国实验方剂学杂志 2013年19卷第22期页码：151-153
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- DOI：10.11653/syfj2013220151
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- 纸质出版日期：2013
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闵春艳, 付妍, 张亚双, 等. HPLC测定小活络丸(浓缩丸)中11-羰基--乙酰乳香酸[J]. 中国实验方剂学杂志, 2013,19(22):151-153.

MIN Chun-yan, FU Yan, ZHANG Ya-shuang, et al. Determination of Acetyl-11-keto--boswellic Acid in Xiaohuoluowan (Concentrated Pills) by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 151-153.
闵春艳, 付妍, 张亚双, 等. HPLC测定小活络丸(浓缩丸)中11-羰基--乙酰乳香酸[J]. 中国实验方剂学杂志, 2013,19(22):151-153. DOI： 10.11653/syfj2013220151.

MIN Chun-yan, FU Yan, ZHANG Ya-shuang, et al. Determination of Acetyl-11-keto--boswellic Acid in Xiaohuoluowan (Concentrated Pills) by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 151-153. DOI： 10.11653/syfj2013220151.

摘要

目的: 建立小活络丸(浓缩丸)中11-羰基-β-乙酰乳香酸的高效液相色谱含量测定方法。方法: Inertsil ODS-3 C18(4.6 mm×250 mm

5 μm)色谱柱

流动相乙腈-0.1%冰醋酸 (80:20)

流速1 mL·min-1

检测波长249 nm

柱温30 ℃

外标法测定小活络丸(浓缩丸)中乳香主成分11-羰基-β-乙酰乳香酸。结果: 11-羰基-β-乙酰乳香酸在25.95~207.6 mg·L-1线性关系良好(R2=0.999 9);平均加样回收率为99.95%

RSD 3.29% (n=6)。结论: 方法操作简便

结果准确

重复性良好

可用于小活络丸(浓缩丸)中乳香的质量控制。

Abstract

Objective: To establish a high performance liquid chromatography (HPLC) method to determine acetyl-11-keto-β-boswellic acid in Xiaohuoluowan (concentrated pills). Method: The determination was carried out with Inertsil ODS-3 C18(4.6 mm×250 mm

5 μm) column. The mobile phase consisted of acetonitrile-0.1% acetic acid (80: 20). The flow rate was 1 mL·min-1. The detection wavelength was set at 249 nm. The column temperature was set at 30 ℃. The external standard method was used to quantify acetyl-11-keto-β-boswellic acid

the main chemical constituent of olibanum in Xiaohuoluowan (concentrated pills). Result: There was a good linear relationship between the absorption peak area and the concentration in the range of 25.95-207.6 mg·L-1(R2=0.999 9) for acetyl-11-keto-β-boswellic acid. The average recovery was 99.95% (n=6). Conclusion: The method is simple

accurate and reproducible. It can be used for quality control of olibanum in Xiaohuoluowan (concentrated pills).

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