Objective: To observe the effect of Puren Dan (PRD) recipe on the apoptosis of INS-1 cell and expression of Bal-xl/Bcl-2 associated death promoter (BAD) and forkhead box O1 (FOXO1) in high glucose condition

and explore the molecular mechanism of PRD on restoring secretion function of INS-1 cell. Method: Experimental injured INS-1 cell model was induced by high glucose (containing 33.3 mmol·L-1)

and intervened by PRD drug-serum. INS-1 cells were divided into 5 groups:the control group

high glucose group

high PRD drug-serum group

low PRD drug-serum group and metformin drug-serum group. Cell viability

INS-1 cell apoptosis and expression level of related proteins and its phosphorylation were detected by CCK-8 assay

flow cytometry and AnnexinV-FITC/PI staning and Western blotting assay

respectively. Result: High glucose could decrease cell viability and induce INS-1 cell apoptosis

and it also downregulatedf phosphorylation level of BAD and FOXO1. PRD can increase cell viability

reduce apoptosis of INS-1 cell downregulated expression of FOXO1 and improved phosphorylation level of BAD and FOXO1. Conclusion: PRD drug-serum shows a protective effect on apoptosis INS-1 cell which induced by high glucose

and the mechanism may be associated with increase of the phosphorylation levels of BAD and FOXO1.