Objective: To study the effect of Danggui Buxue decoction on proliferation in the hypoxic vascular endothelial cells and to explore the mechanism of angiogenesis. Method: CCK-8 assay was used for the proliferation of vascular endothelial cells;ELISA assay was used for vascular endothelial growth factor (VEGF) and its receptors (VEGFR1

VEGFR2

sVEGFR1

sVEGFR2) expression of vascular endothelial cells. Result: Compared with normal group

the hypoxic endothelial cell proliferative capacity was significantly decreased (P<0.01).Danggui Buxue decoction could promote the proliferation of normal endothelial cells and hypoxic endothelial cells with dose-response relationship compared with model group (P<0.01). Danggui Buxue decoction could promote expression of VEGF in hypoxic vascular endothelial. Compared with model group

Danggui Buxue decoction could significantly up-regulate VEGF

VEGFR1

VEGFR2 expression

and showed a dose relationship

but could inhibit sVEGFR1

sVEGFR2 expression. Conclusion: Danggui Buxue decoction can promote proliferation of the hypoxic vascular endothelial cells

The mechanism might be related to regulation of VEGF and its receptors.