Objective: To study the inhibitory effect of Yulangsan polysaccharide (YLSP) on duck hepatitis B virus (DHBV) persistent infection in the model for the serum DHBV-DNA and the protecting effect on hepatocytes. Method: Ducklings were infected with DHBV and randomly divided the 3YLSPS dose groups(10

5

2.5 g·kg-1)

lamivudine (3TC

0.05 g·kg-1) and model groups respectively

continuous administration for 14 days. The serum content of DHBV-DNA was determined by real time fluorescent quantity polymerase chain reaction(FQ-PCR) method

DHBsAg and DHBeAg in serum were detected by ELISA before treatment and on the 7th

14th day after treatment and At 3 days after stopping treatment

took the liver homogenate and detected in liver and serum superoxide dismutase(SOD)

glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)

L-glutathione (GSH) content. Result: Compared with modle group

the serum levels of DHBV-DNA

DHBsAg and DHBeAg decreased significantly 7 and 14 days after treatment with high-dose and middle-dose YLSP and at 3 days after stopping treatment the DHBV-DNA

DHBsAg and DHBeAg in serum in high-dose group were not rebounded

in liver and serum SOD

GSH-Px activity and MDA

GSH content

high-dose and middle-dose YLSP could still show a sustained and effective

no rebound phenomena (P < 0.05 or P < 0.01). Conclusion: YLSP can inhibit replication of DHBV and protect hepatocytes.