Objective: To study effect of portalaca total flavone(PTF)on H9c2 myocardial cell inj induced by anoxia/reoxygenation (A/R)and its possible mec hanism. Method: Myocytes cell model of A/R injury was establis hed in vitro cultured.The cell viability was measured with MTT

Intra cellular calcium concentration was determined by Fluorospectrop hotometry

nitric oxide(NO) concentration were detected using colori metricmet hod

using flow cytometry to detect myocardial cell apoptosis rate

myocardial cell apoptosis gene expression of Caspase-3 mRNA detected by RT-PCR. Result: Compared with the A/R group

after pretreatment with 10

20 and 40 mg·L-1 PTF

PTF groups can significantly improve anoxia/reoxygenation injury of myocardial cell survival rate

reduce the concentration of NO

intracellular calcium ion concentration

the cell apoptosis rate (P<0.05

P<0.01)

PTF groups can effectively reduce hypoxia/reoxygenation injury of myocardial Caspase-3 mRNA expression in the cell. Conclusion: PTF can protect the myocardial cells with in hibit myocardial intracellular calcium overload

reducing excessive NO cytotoxic effect and downgrading of caspase 3 mRNA expression.