补中益气汤对裸鼠肺腺癌移植瘤PI3K/AKT信号通路作用的研究

刘亚莉; 唐莹; 易佳丽; 井欢; 刘春英

doi:10.11653/syfj2013220232

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补中益气汤对裸鼠肺腺癌移植瘤PI3K/AKT信号通路作用的研究

更新时间：2024-01-04

- 补中益气汤对裸鼠肺腺癌移植瘤PI3K/AKT信号通路作用的研究
- Experimental Research on PI3K/AKT Expression Mechanisms of Buzhong Yiqi Decoction in Nude Mice Lung Adenocarcinoma Transplantation Tumor
- 中国实验方剂学杂志 2013年19卷第22期页码：232-235
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81072743);辽宁省教育厅资助项目 (L2010355)
- DOI：10.11653/syfj2013220232
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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刘亚莉, 唐莹, 易佳丽, 等. 补中益气汤对裸鼠肺腺癌移植瘤PI3K/AKT信号通路作用的研究[J]. 中国实验方剂学杂志, 2013,19(22):232-235.

LIU Ya-li, TANG Ying, YI Jia-li, et al. Experimental Research on PI3K/AKT Expression Mechanisms of Buzhong Yiqi Decoction in Nude Mice Lung Adenocarcinoma Transplantation Tumor[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 232-235.
刘亚莉, 唐莹, 易佳丽, 等. 补中益气汤对裸鼠肺腺癌移植瘤PI3K/AKT信号通路作用的研究[J]. 中国实验方剂学杂志, 2013,19(22):232-235. DOI： 10.11653/syfj2013220232.

LIU Ya-li, TANG Ying, YI Jia-li, et al. Experimental Research on PI3K/AKT Expression Mechanisms of Buzhong Yiqi Decoction in Nude Mice Lung Adenocarcinoma Transplantation Tumor[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 232-235. DOI： 10.11653/syfj2013220232.

摘要

目的: 探讨补中益气汤对裸鼠肺腺癌移植瘤生长抑制作用及对磷脂酰肌醇3 激酶(phosphatidylinositol 3 kinase

PI3K)/蛋白激酶 B(protein kinase B

AKT)信号通路的影响。方法: 50只裸鼠随机分为①正常对照组

②荷瘤对照组

③顺铂组

④顺铂联合补中益气汤组

⑤补中益气汤组。分别给予相应干预后

处死小鼠

计算抑瘤率;应用免疫组化方法、实时定量 PCR(real time PCR

RT-PCR)检测移植瘤组织中PI3K

AKT的表达水平。结果: 顺铂联合补中益气汤组对移植瘤的抑制率最强

与其他各组比较有显著差异(P<0.05)

顺铂和补中益气汤协同作用的q值1.201;移植瘤中PI3K

AKT蛋白和mRNA基因的表达在顺铂联合补中益气汤组显著降低(P<0.05)。结论: 补中益气汤联合顺铂对耐顺铂肿瘤有协同抑制作用;补中益气汤能通过干预PI3K、AKT基因和蛋白的表达而增加顺铂对耐顺铂肿瘤的敏感性。

Abstract

Objective: To discuss the effect on lung adenocarcinoma transplantation tumor in nude mice and the influence of phosphatidylinositol 3 kinase/protein kinase B(PI3K/AKT)signaling pathway of Buzhong Yiqi decoction. Method: 50 nude mice were randomly divided into 5 groups

intervented respectively after 14 days

put to death and calculating tumor inhibition rate and used immunohistochemical method and real time PCR(RT-PCR) detecting the expression level of PI3K

AKT in transplantation tumor tissue. Result: Conbined group was the strongest inhibition rate of transplanted tumor

compared with other groups

there are statistically significant (P<0.05). The q value of synergistic effect of cisplatin and Buzhong Yiqi decoction was 1.201. PI3K and AKT protein and gene mRNA expression in conbined group were decreased. There are statistically difference among other groups (P<0.05). Conclution: The Buzhong Yiqi decoction combined with cisplatin has synergistic inhibitory effect in Cisplatin resistance tumor.The Buzhong Yiqi decoction can increase cisplatin resistance mechanism throught regulating the gene and protein expression of PI3K and AKT.

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