Objective: To evaluate the toxicity of imcompatible pair of Sargassum and Radix Glycyrrhizae in mice by comparing the acute toxicity of different Haizao Yuhu decoction groups. Method: The mice was given different combination and compatibility of Haizao Yuhu decoction with different doses by intragastric administration

and calculated the LD50. If the group didn't meet the LD50 evaluation criteria we would give the drug by continuous intragastic administration for 28 days

ie

we would evaluate the toxity by caculating cumulative median lethal dose. The blood of mice was collected by picking bulb of eye. Detected relevant indicators of liver and kidney function in mice serum to evaluate their influence on liver and kidney. Result: The LD50(n) of the Haizao Yuhu decoction(Radix Glycyrrhizae) is 71.08 g·kg-1

the LD50(n) of the Haizao Yuhu decoction (the prepared Radix Glycyrrhizae) is 68.28 g·kg-1

the LD50(n) of the Haizao Yuhu decoction removed Radix Glycyrrhizae is 55.78 g·kg-1

the LD50(n) of the Haizao Yuhu decoction removed Sargassum(Radix Glycyrrhizae) is 72.27 g·kg-1

the LD50(n) of the Haizao Yuhu decoction removed Sargassum and Radix Glycyrrhizae is 79.62 g·kg-1. The Haizao Yuhu decoction removed Sargassum (the prepared Radix Glycyrrhizae) still didn't reach median lethal dose after continuous administration for 28 days

the maximum tolerated dose of this group is 80 g·kg-1;each experimental groups were not showed a significant impact on the liver and kidney functions in mice. Conclusion: The LD50(n) of each experimental group belongs to non-toxic category

and each group has no significant impact on the liver and kidney system in mice.