Objective: To explore mechanism of the liver injury caused by giving Eudiae Fructus evodiae decoction in mice. Method: KM mice were divided into four groups:normal control

high

middle and low dose groups which were administrated respectively with distilled water or equal volume of Euodiae Fructus decoction at the doses of crude drug 30

20

10 g·kg-1 daily by gavage for consecutive 21 days. The blood of the animals was collected by pulling out the eyeball. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automatic biochemical analyzer. The livers were collected. The liver weight indexes were calculated and liver pathological changes were observed. The activity of superoxide dismutase (SOD)

glutathione (GSH)

glutathione peroxidase (GSH-Px)

nitric oxide synthase (NOS)and the contents of malonaldehyde (MDA)in liver samples were measured by Kit. The ratio of SOD/MDA was calculated. The contents of interleukin-1β(IL-1β)

interleukin-6(IL-6)

tumor necrosis factor-α(TNF-α) in liver samples were detected by ELISA. Result: Compared with control group

the activities of ALT and AST were significantly increased in high

middle and low dose groups. The liver histopathological change was characterized by emergence of focal necrosis of liver cells. The activity of GSH-Px

NOS and the ratio of SOD/MDA in the liver were significantly decreased (P<0.05)

the tissue content of GSH was increased (P<0.05)

the contents of IL-1β

IL-6

TNF-α of the liver were significantly increased(P<0.01). Conclusion: The mechanism of the liver injury caused by high dose of Euodiae Fructus decoction in mice may be related to oxidative stress and inflammatory response.