Objective: This study tries to approach the long-term toxicity of Radix Aconiti (RA) combined with Fructus Trichosanthis (FT) in rats

and investigate the relationship between compatibility and toxicity about this anti-drugs. Method: Eighty male and female SD rats were randomly divided into RA 0.3 g·kg-1

RATF 0.3

0.1 g·kg-1 and the contrast groups

each group having 10 male rats and 10 female rats. the rats of the drug administered groups received drugs orally with the water extract of RA and RAFT

the oral volume was 5 mL·kg-1

the rats were administered one time a day for 30 days. 24 hours later after the last time of drug administration

2/3 of the rats were sampled blood from their abdominal aorta and organs to figure the indexes of organs out

detecting heart creatine kinase(CK)

lactate dehydrogenase(LDH)

liver aspartate aminotransferase(AST)

alanine aminotransferase(ALT)

kidney blood ureanitrogen(BUN)

creatinine(Cr) and other biochemical markers

making the pathological examination for Major tissues and organs. The remaining 1/3 rats were detected the corresponding indicator in the same way 15 days later after stopping drug administration. Result: The kidney index of female rats in every drug administered groups was higher than contrast group's and the thymus index was lower. The RBC were higher than the contrast group (P<0.05). The AST

ALT

LDH and BUN of each drug administered group were higher than the contrast group (P<0.05

P<0.01)

and the Cr of female rats was higher than the contrast group in RA 0.3 g·kg-1 and RAFT 0.3 g·kg-1 groups(P<0.05

P<0.01). The AST

LDH of female rats in RA 0.3 g·kg-1 group were higher than ARTF 0.1 g·kg-1 group (P<0.05

P<0.01). The AST

ALT

Cr

LDH of female rats and the AST of male rats in RAFT 0.3 g·kg-1 group were higher than RAFT 0.1 g·kg-1 group (P<0.05

P<0.01). A certain extent visceral injury can be seen in heart

liver

and kidney of the rats in RAFT 0.3 g·kg-1 group through main viscera conventional HE dying. The AST

ALT of female rats and the ALT of male rats in RA 0.3 g·kg-1 and RAFT 0.3 g·kg-1 group were higher than contrast group at the end of recovery term. The differences of AST

ALT in AR group and ARTF group were not statistically significant. Conclusion: Radix Aconiti has significant toxicity in heart

liver and kidney of rats in the given dose. The long-term toxicity has no obvious difference between RA group and RAFT group in rats

but more harmful in female rats than in male.