Objective: To optimize separation and purification technology of total flavonoids from ethanol extract of Ziziphi Spinosae Semen(ZSS) by macroporous adsorption resin. Method: Adsorption and desorption properties of six different types of macroporous resins for total flavonoids from ZSS were compared by static and dynamic adsorption and desorption tests to select optimum type of macroporous resin;With the content of total flavonoids as index

single factor tests were adopted to investigate adsorption and elution conditions of macroporous resin

such as the concentration of sample diluted

adsorption rate

eluent concentration and so on. Result: SP-207 macroporous resin was adopted

optimum separation and purification conditions for total flavonoids from ZSS were as follows:the concentration of sample solution 0.5 g· mL-1

adsorption rate 1 BV· h-1

sample volume 5 BV

eluted by 4.5 BV of 70% ethanol at a flow rate of 4 BV· h-1;The content of total flavonoids from extract of ZSS was 65.47%. Conclusion: This optimized process was stable

feasible and suitable for separation and purification of total flavonoids from ZZS.