Objective: To optimize purification process of total triterpenoid acids from Elaeagnus bockii by macroporous resin. Method: The content of total triterpenoid acids was determined by UV.Type of macroporous resin was screened by dynamic adsorption-elution test;With the content of total triterpenoid acids as index

influence factors were selected by single factor tests

main factors were investigated by Plackett-Burman.Taking composite score of the content of total triterpenoid acids and extract yield as index

L9(34) orthogonal test was applied to optimize purification process of total triterpenoid acids with ethanol concentration

alcohol amount

ratio of ethanol and 1% sodium hydroxide as factors. Result: Optimum purification process conditions were as follows:eluent amount 4 BV

ratio of 80% ethanol and 1% NaOH (1:5)

flow rate 3 mL· min-1;The mass fraction of total triterpenoid acids was 75%

extract yield was more than 22%. Conclusion: This optimized process was simple and stable to provide experimental basis development and utilization of total triterpenoid acids from E. bockii with high precision and predictability.