Objective: To optimize microwave extraction process of effective parts in Caulis Polygoni Multiflori for improving sleep. Method: Single-factor tests were adopted to investigate effects of extraction time

ethanol concentration

microwave power

solvent amount and extraction temperature on contents of total flavonoids and total anthraquinones in Caulis Polygoni Multiflori.Based on single factor tests

taking extraction time

ethanol concentration and microwave power as factors

each factors was set five levels

with the "Overall Desirability" of contents of total flavonoids and total anthraquinones as evaluation indicator

then extraction process of effective parts in Caulis Polygoni Multiflori for improving sleep was optimized by central composite design-response surface methodology. Result: Optimum microwave extraction conditions were as follows:microwave power 585 W

extracted 11 min with 10 times the amount of 82% ethanol at 60℃

extracted 1 time. Conclusion: Optimized extraction technology was simple and feasible with a good predictability

it could provide experimental evidence for formulations development and industrial production of Caulis Polygoni Multiflori.