Objective: To optimize formulation of Jiangjiao gels and investigate its in vitro percutaneons penetration. Method: Taking cumulative permeation amount per unit area in 24 h of capsaicin as index

effect of dosages of glycerol

propylene glycol and azone on formulation process of capsaicin was investigated by orthogonal test;With isolated rat skin as transdermal barrier

Franz diffusion cell was used as in vitro percutaneous release model

then in vitro transdermal permeability of capsaicin was investigated.The content of capsaicin was determined by HPLC

chromatographic conditions were as follows:Diamonsil C18 column(4.6 mm×250 mm

5 μm)

mobile phase of methanol-0.1% phosphoric acid solution(65:35)

flow rate 1.0 mL· min-1

detection wavelength 280 nm

column temperature 40℃

injection volume 20 μL. Result: Optimal formulation of Jiangjiao gels was 2% carbomer

10% glycerol

20% propylene glycol and 1% azone;In vitro transdermal permeation of capsaicin in Jiangjiao gels fitted zero order kinetics equation

percutaneous permeation rate was 0.353 μg· h-1· cm-2. Conclusion: Optimized formulation was reasonable and suitable for topical skin.