Objective: To optimize purification technology conditions of total flavonoids from leaves of Broussonetia papyrifera by macroporous adsorption resin. Method: With total flavonoids from leaves of B. papyrifera as index

type of macroporous resin was screened by by static adsorption-desorption and dynamic adsorption-desorption tests

effects of eluent consumption

elution flow rate

the concentration of sample solution and other factors on purification technology were investigated by single factor tests. Result: HPD450 was selected for its excellent adsorption and desorption properties

optimum process was as following:the concentration of sampling solution 4.4 g· L-1

sampling velocity 1 BV· h-1

sample liquid volume 6 BV

eluted by 5 BV deionized water and 8 BV 50% ethanol at speed of 1 BV· h-1

diameter to height ratio of resin 1:8

collected eluate.Under these conditions

purity of total flavonoids was up to 75.75% from 30.65%

retention rate of total flavonoids reached 79.89%

impurity removal rate reached 64.88%. Conclusion: HPD450 macroporous resin was suitable for separation and purification of total flavonoids from leaves of b. papyrifera

optimized purification technology was stable and feasible

it could be extended for industrial production applications.