Objective: To optimize purification process of total polyphenols from acid Granti Pericarpium in Kashi. Method: With the content of total polyphenols as index

type of macroporous resin was screened by static adsorption-elution test

single factor tests were adopted to optimize purification process of total polyphenols by taking sample solution concentration

eluent consumption

adsorption speed and others as factors. Result: HPD500 macroporous resin was selected

optimum purification process was as follows:the crude drug concentration of sample solution 20.8 g· L-1

pH of sample solution 2

sample volume 8 BV

adsorption rate 2 BV· h-1

adsorption time 12 h

eluted impurities with 7 BV of distilled water and 8 BV of 50% ethanol

collected ethanol eluate;Purity of total polyphenols was up to 66.93% from 24% before purification

yield of extract was 34.80%. Conclusion: HPD500 macroporous resin was suitable for purification of total polyphenols from acid Granti Pericarpium

optimized process conditions were simple and feasible with high efficiency and low toxicity.