Objective: To determine the content of gallic acid and ellagic acid and establish fingerprints to assess the quality of Phyllanthi Fructus derived from thirteen batches of Phyllanthus emblica

which is in order to establish the quality evaluation of Phyllanthi Fructus. Method: HPLC fingerprinting and content determination methods were applied to evaluate thirteen batches of Phyllanthi Fructus. The samples were separated by an Alltima C18 column (4.6 mm×250 mm

5 μm) using acetonitrile-0.2% phosphoric acid with water gradient system as mobile phase.The flow rate was 1.0 mL· min-1

the detection wavelength for fingerprinting was at 273 nm

and the wavelength for content determination of gallic acid and ellagic acid were at 273 nm and 254 nm respectively. Result: HPLC fingerprint of Phyllanthi Fructus was established with good separation and repeatability

which could be used for quality assessment of Phyllanthi Fructus. The results showed that the HPLC fingerprints were similar among thirteen batches samples of Phyllanthi Fructus

but significantly different between Hong Kong samples and mainland samples. The content of gallic acid and ellagic acid from Hong Kong and mainland was obviously different from each other. The HPLC fingerprints of herbal Phyllanthi Fructus and ripe fruity of P. emblica were similar

while the content of gallic acid and ellagic acid was obviously different. Conclusion: The method is sensitive

repeatable and accurate

it can be used as quality control for Phyllanthi Fructus and has been adopted in "Hong Kong Chinese Materia Medica Standards".The content of gallic acid and ellagic acid varied significantly in Phyllanthi Fructus from different habitats which suggested that more attention should be paid to clinical use of different Phyllanthi Fructus (including herbal Phyllanthi Fructus and ripe fruity of P.emblica) in practice.